Coding

Part:BBa_K4855000

Designed by: Slavil Peykov   Group: iGEM23_Bulgaria   (2023-10-11)


blaNDM-2&5

This part comprises the coding sequence of a synthetic hybrid MBL enzyme capable of degrading all beta-lactams except monobactams. It incorporates all the mutations found in the NDM-2 and NDM-5 variants, which were identified as having the highest activity against meropenem based on literature data. The iGEM Bulgaria 2023 team employed this component in their enhanced CADABRA cocktail.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None